Background: Adolescents form precious human resources in every country & constitute 18% of the total population in India.The majority of adolescents lack basic knowledge about puberty, growth during puberty, safe sex, and hygiene. Adolescence is a period of rapid physical growth, sexual and psychological changes. Aim: The aim of the study was to understand the knowledge, attitude regarding reproductive health and to assess the practices regarding reproductive health among school-going adolescent girls of 6th to 10th standard. Methods: It is a cross-sectional study carried out among school-going adolescent girls of standard 6 to 10 in the field practice area of a medical college in Hyderabad. The study duration was from February 2022 to January 2023. The data collection tool used for the study was a semi-structured questionnaire. The data was analysed by using SPSS 20 software. Results: Among the total of 904 participants 45.7% were scared during the first menstrual cycle. Dysmenorrhoea was most common in class IV socioeconomic status (53.7%).31.4% of study participants had occasional itching and 5.3% had continuous itching. About 10.6% of study participants consulted doctors during menstruation of which the majority of them belonged to 10 to 12 years (16.3%). 58.6% of study participants did household activities during menstruation of which the majority were studying 9th class (69.2%). Conclusion: The present study concluded that female adolescents had unsatisfactory knowledge, inadequate hygiene practices, and positive attitudes toward Reproductive Health.