Abstract Issue

Volume 12 Issue 1 (January- March) 2023

Original Articles

A study on laboratory findings of SarsCov 2 viral RNA parameters in Saliva and Respiratory Specimens in COVID 19 Patients
Sarasa. S, Rathinam Suresh

Background:Reverse Transcription Polymerase Chain Reaction of respiratory samples is the diagnostic test for detecting the SARS CoV 2 infection. The nasopharyngeal and oropharyngeal swab was considered to be the best specimen for the diagnosis of the COVID 19. But the invasiveness of the procedure can reduce the likelihood of the patient permission to retest and can pose a significant risk for the treating healthcare workers. Hence the present study focusses on the viral shedding rate in the saliva, nasopharyngeal and oropharyngeal sites to propose some practical suggestions regarding the efficacy of the samples in the detection of SARS CoV 2. Methods:The samples were collected from the patients with or without symptoms after 5 to 7 days of their positivity. Three respiratory samples such as Saliva, Nasopharyngeal swab and Oropharyngeal swab were taken separately at the same time. The detection of SARS-CoV-2 in the specimens was performed by RT-PCR amplification of the SARS-CoV-2 RdRP and N gene fragments, using a LabgunExofast RTPCR kit. The detection of human RNase P gene was included in the kit as a control. The test was performed in the QuantStudio™ 5 Real-Time PCR System. The result was interpreted as positive when the cycle threshold (Ct) values of both target genes were less than 30 cycles.Result& Discussion:In this study, we proved saliva as an acceptable non-invasive alternative source for the diagnosis and viral load monitoring of SARS-CoV-2 in a large cohort of patients. Saliva exhibited comparable sensitivity and strong agreement to the current COVID-19 diagnosis standard by using respiratory tract specimens. In summary, our study showed that saliva might serve as a promising substitutable choice to the current COVID- 19 diagnosis standard by using respiratory tract specimens with comparable performance.

 
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