Background: Vulvovaginal candidiasis (VVC) is the most common vaginal infection affecting women of reproductive age. The present study aimed to assess the prevalence of VVC and associated risk factor for VVC, and antifungal susceptibility testing profile of Candida species causing VVC. Methods: This hospital-based prospective observational study included suspected cases of VVC, in married and sexually active women. The clinical and demographic data such as age, parity, oral contraceptive pills (OCPs) uses, antibiotic profile and diabetic history were recorded. Results: A total of 130 patients were included in the study, out of which the majority (54.6%) of women were in the 18-30 years of age group. A totalof 81.5% of women had vaginal discharge, whereas 14.6% of women had recurrent discharge. The patients from urban areas had a significantly higher proportion of Candida positive isolates than negative isolates [96.0% vs 69.9%; P=0.0001]. The patients who had OCPs had higher chances of development of Candida-positive isolates as compared to negative isolates [12.0% vs 3.0%; P=0.06]. Antibiotic use was associated with an increased risk of colonization by Candida species (44.0%). Biofilm production was majorly observed among patients who were positive for Candida glabrata and Candida albicans isolates. Fluconazole showed 24.0% resistance and 76.0% sensitivity, itraconazole showed 76.0% resistance and 24.0% sensitivityandvoriconazoleexhibited no resistance with 100.0% sensitivity. Conclusion: The VVC diagnosis was associated with antibiotic prescriptions and diabetes and pregnancy. The antifungal susceptibility result showed that all candida species showed higher sensitivity towards voriconazole treatment.