Background: Disorders of the upper gastrointestinal tract are frequent symptoms in clinical practice and are associated with high rates of death and morbidity The present study evaluated histomorphological spectrum of upper gastrointestinal lesions. Materials & Methods: 76 upper GIT endoscopic biopsies of both genders underwent standard tissue processing, including fixing in 10% buffered formalin, cutting tissue into sections that were 3–4 microns thick, and staining with hematoxylin and eosin. Acid-Fast and periodic Acid Schiff (PAS) staining were carried out. Results: Out of 76 cases, males were 40 and females were 36. Out of 24 neoplastic lesions, 8 belonged to esophagus, 4 stomach and 2 duodenum. Out of 52 non- neoplastic lesions, 6 were of esophagus, 14 were of stomach and 18 were of duodenum. The difference was significant (P< 0.05). There was correlation of endoscopic and histopathological findings of oesophageal lesions (P< 0.05). Conclusion: An efficient investigative technique for evaluating patients with upper gastrointestinal symptoms and obtaining representative biopsies is upper gastro- intestinal endoscopy. Upper GI endoscopy helps in early detection of mucosal lesions, diagnosis of the carcinoma at early stage and confirmation of clinically suspected cases leading to early clinical management.