Background: Dermatophyte is a group of saprophytic fungi(1). Several members of this distinct group of fungi have evolved to cause superficial mycoses. Aim and Objective: Mycological study on spectrum of tinea infection and immune status of patients attending tertiary care hospital, Bundelkhand Medical College Sagar. Material and Methods: Clinical samples viz. hairs, nails, and skins were collected from 179 patients. A portion of each sample was examined microscopically, and the remaining portion of each sample was cultured. Dermatophyte isolates were identified by studying macroscopic and microscopic characteristics of their colonies. Result: Out of 179 samples, dermatophytes were detected in 126 (70.3%) cases. The most common species of dermatophyte identified on culture was Trichophyton rubrum (42.9%), followed by others. Tinea corporis was the predominant clinical manifestation accounting for 25% of the cases .A total of 116 (65.1%) participants were using steroids for one or more reasons. Conclusion: Most cases of dermatophytosis were caused by Trichophyton rubrum and were associated with topical steroid use.