Background: Early initiation of treatment and early diagnosis of sepsis can help in preventing the progression of sepsis to septic shock and severe sepsis. Considering a continuous increase in the severity and rate of maternal sepsis globally and limited literature data showing the actual burden of maternal sepsis. Aim: The present study was designed to assess the specificity and sensitivity of different biomarkers in subjects diagnosed with sepsis and admitted to the ICU of the Obstetrics Department with the diagnosis of sepsis and compared biomarkers to clinical criteria in these subjects. Methods: The study assessed 104 subjects admitted to critical care of the obstetrics department with a diagnosis of sepsis and clinically assessed on SOFA and q SOFA scores and with quantitative biomarkers assessment for sepsis including procalcitonin, serum lactate, and CRP. Also, the specificity and sensitivity of both biomarkers and clinical criteria were studied. Results: Among biomarkers of subjects diagnosed with sepsis, a high specificity and sensitivity were seen for serum lactate and procalcitonin. A low specificity of 57.58% and a high sensitivity of 78.7% were seen with the q SOFA scores in the present study, whereas, total SOFA scores showed a high specificity of 84.78% depicting the role of total SOFA scores as confirmatory criteria and q SOFA as a screening-criteria in subjects with sepsis. Conclusion: The present study concludes that the most specific and sensitive predictor for prognosis in subjects with maternal sepsis is serum lactate along with procalcitonin, whereas, the least specific biomarker for maternal sepsis is CRP. Total SOFA and q SOFA scores can be used for early screening of maternal sepsis.