Background:Tuberculosis, an infectious disease caused by Mycobacterium tuberculosis, remains a major threat to public health. TB is usually divided into two main categories depending on the MTB infection site: pulmonary and extrapulmonary tuberculosis. The elimination of Tuberculosis requires early, rapid and accurate diagnosis and treatment. Acid-fast bacilli microscopy with ZN staining is the most frequent laboratory technique used for its diagnosis. A culture technique using Lowenstein–Jensen medium for mycobacterial growth is being considered as the gold standard method for TB detection and it takes a longer time which is usually 3–4weeks with a high sensitivity.Methods:A total of 500 specimens were collected during this study from suspected Pulmonary and Extrapulmonary tuberculosis cases from different wards and outpatient departments Patients with known clinical diagnosis other than the disease, patients already on ATT, and patients with HIV infection were excluded from the study.Result:Out of 205 pulmonary and 295 extrapulmonary suspected tuberculosis patients, 32 were positive from pulmonary tuberculosis patients While in extrapulmonary tuberculosis patients 28 were positive.Conclusion:The efficiency of MTB culture using the LJ medium a high sensitivity and has been demonstrated to detect MTB when 10 viable bacilli per mL of sputum were present.