Abstract Issue

Volume 12 Issue 4 ( October-December ) 2023

Original Articles

Investigation of Dermatophytosis at a Tertiary Healthcare Facility: A Clinico-Mycological Analysis
Dr. Nupur Shanker, Dr. Tejaswini Shekhar Sharma

Background: Dermatophytosis encompasses a collection of surface-level fungal infections affecting keratinized tissues, including the epidermis, hair, and nails. This research aims to explore the clinical characteristics of dermatophytosis and pinpoint the fungal species responsible for diverse clinical manifestations. Methods: Conducted within a hospital setting, this observational study involved the inclusion of 200 patients with clinically suspected dermatophytosis. The methodology encompassed a comprehensive approach, including the collection of patient histories, general physical examinations, and detailed assessments of skin lesions. Diagnostic procedures involved direct microscopy using 10% KOH (40% KOH for nail specimens) and fungal culture on Sabouraud Dextrose Agar (SDA) supplemented with 0.05% chloramphenicol and 0.5% cycloheximide for every participant. Statistical analysis utilized the Chi-square test and contingency coefficient test to establish significance in the data. Results: In the current investigation, tinea accounted for 22.68% of all cases, with males comprising 59% and females 41% of these cases. The average age of individuals affected was 38 years, with the majority falling within the 21-30 years age group, followed by the 31-40 years age group. Among the collected samples, 136 cases were positive in the potassium hydroxide (KOH) test, while 122 cases showed positive results in the fungal culture. Interestingly, four cases that were KOH negative turned out to be culture positive, and 18 cases negative in culture were found to be KOH positive.In the subset of 162 skin scraping samples, 108 were positive in the KOH test, and 94 were positive in the fungal culture. Among the 22 nail clipping samples, 16 were positive in both the KOH test and culture. Additionally, in the 16 hair pluck samples, 12 were positive in both the KOH test and culture. Conclusion: Dermatophytosis stands out as one of the most prevalent, if not the most widespread, dermatological conditions encountered. Its impact is notably pronounced in the younger demographic, showing a predilection for affecting individuals in the younger age groups rather than at the extremes of age.

 
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