Background: Exposure to antibiotics in childhood is thought to alter the intestinal microbiome. Most of the data in literature are from case reports and small-sample cross-sectional studies; however, several recent short-term longitudinal studies have provided estimates of the incidence of adverse health events after enteric infections. Hence; under the light of above-mentioned data, the present study was undertaken for assessing the microbiological profile of pediatric patients with infected gut.Materials &Methods: A total of 50 pediatric subjects within the age range of 6 to 16 years were enrolled. Complete demographic and clinical details of all the subjects were obtained. Inclusion criteria for the present study included pediatric subjects admitted because of gut infection. Subjects with presence of any other systemic illness, or any known drug allergy were excluded from the present study. Stool samples were collected prior to discharge home. All the samples were sent to microbiological department where Blood agar and Macconkey agar was used followed by incubation at 37 degrees centigrade for overnight with the aim of evaluating the microlocal growth. Quantification of the microbial growth was done.Results:A total of 50 subjects were analyzed. Mean age of the subjects was 10.3 years. 66 percent of the subjects were boys while the remaining were girls. Neutrophil count and lymphocyte count was 15.32 (x103/µL) and 2.13 (x103/µL)respectively. Salmonella was the main organism identified, found to be present in 46 percent of the cases. E.coli was found in 20 percent of the cases. Actinobacteria and Bacteroides were identified in 14 percent and 10 percent of the cases. Conclusion:In children presenting for emergency department care with acute gut infection, Salmonella was the main bacterium responsible for it.