Introduction- A number of infectious pathogens can cause hepatitis, an inflammation of the liver. One of the causes of hepatitis is hepatitis C, which can result in hepatocellular cancer. Using serological techniques to detect antibodies, an infection's diagnosis is made. The aim of present study is to study the prevalence of HCV infection in tertiary care hospital at GBCM and Subharti Hospital, Dehradun (UK), India. Material and methods- The present prospective study was conducted at department of microbiology among patients who visited to OPD & IPD of Subharti hospital during the period of July 2022 to July 2023. Total 3833 test were conducted. Demographic of patients were noted and results were analyzed using SPSS version 25.0. Results – Out of total 3833 test conducted only 44 tests came positive depicting the prevalence of HCV as 1.14%. Most of patients were from the age group of 31 to 45 years (29.5%) followed by 46 to 60 years (25%) and 16 to 30 years (22.7%), 61 to 75 years (22.7%) . Out of all patients 50% were male and 50% were female. 36.3% patients were from general surgery department. Conclusion – In India, hepatitis C is becoming more common. Prevention should focus on providing education, risk reduction counseling, HCV screening, and drug misuse treatment to individuals who are susceptible to contracting the hepatitis C virus.