Quality control in clinical laboratory testing systems has a significant impression to fulfil the expectation of clinicians and patients expectation for disease diagnosis and treatment. Regular runs of internal QC safeguard the precision and accuracy of tests and also conserve the quality of the laboratory. The veracity of internal quality samples is much important to meet the requirements of proficiency testing. The present study was designed to evaluate and monitoring of internal control glucose and total cholesterol in the clinical laboratory of Rama Hospital. The present results of quality control materials showed that Trends (loss of reliability) and shifts (abrupt changes in the control mean) were noted. Warning signs and violations of the rule were recorded and action initiated. The violation of the controls identifies system error and analytical bias, which is not always clinically relevant.