Aim: The aim of the present study was to evaluate the antimicrobial susceptibility pattern of microorganisms involved in the pathogenesis of surgical site infection. Methods: The study was a cross sectional study which was carried in the Department of Microbiology, for the period of one year, after taking the approval of the protocol review committee and institutional ethics committee. Total 380 patients with SSIs either sex or any age, who had surgical wound pus, discharge, or signs of sepsis were include in this study. Patients with cellulitis and suture abscess were excluded from this study. Out of 380 samples, 200 samples were culture positive (52.63%). Results: Among 200 positive samples 110 (55%) were males. Maximum no. of culture positive samples in age 20-30 years (31%) followed by 30-40 (20%) and then followed by 40-50 (15%) of age group respectively. Out of 200 culture positive samples S. aureus (22%) was the most common pathogen isolated followed by Escherichia coli. (22%), Citrobacter spp. (15%) and Pseudomonas aeruginosa (12%) respectively. Among gram negative bacilli, E. coli was most sensitive to Imipenem followed by Amikacin and Piperacillin Tazobactam whereas for Citrobacter spp., Imipenem followed by Gentamicin, Ciprofloxacin was the drug of choice then for Klebsiella spp., Imipene followed by Gentamicin, Amikacin was the drug of choice. Among gram positive organism, S.aureus showed maximum antibiotic sensitivity to Linezolid followed by Vancomycin, Amikacin whereas CONS was sensitive to Linezolid followed by Vancomycin and Gentamicin. Conclusion: The present study concluded that the increasing resistance to antimicrobials increases the risk of morbidity and mortality; therefore, there is urgent need of implementation of measures to restrict the health care associated infection. Rational use of antimicrobials, proper hygiene, and strict asepsis should be applied in all health care.