Background: Epilepsy is a prevalent neurological disorder characterised by recurrent episodes of seizures, with or without convulsions, as well as sensory or psychiatric phenomena.Ocimum sanctum L. commonly known as tulsi (synonym of Ocimumtenuiflorum L.) is widely used in Ayurveda medicine and is having multitude neuromodulatory effect.Current study investigated the protective effect of Ocimum sanctum leaf extract (OSLE) with antiepileptic drug (Carbamazepine) in Pentylenetetrazol (PTZ)- induced convulsions in rats. Methods: This experimental study was conducted on 54 adults healthy Wistar rats. Rats were randomly divided into 9 groups, as mentioned below. Each group contained 6 rats and underwent different treatment regime. They were assessed for 14 days. Results: PTZ induced changes in the oxidative parameters in rats brain was found significantly decreased (p<0.0001, p<0.05) with Ocimum sanctum (500mg/kg and 1000mg/kg) in a dose dependent manner. PTZ(30mg/kg) induced significant decrease in the antioxidant enzymes (SOD). The lipid peroxidation marker (MDA) significantly increases (p<0.05) after PTZ(30mg/kg) in the rats brain which was found reversed by pre-treatment with Ocimum sanctum. The markers of inflammatory cytokines (pro-inflammatory-IL-1β and anti-inflammatory- IL-4) were found significantly increase in the PTZ-treated (30mg/kg)(p<0.0001). Molecular marker (NF-kβ) was significantly increased in all treated group (p<0.0001).The level was suppressed more in Ocimum pre-treated group as compared to the vehicle. Among neurobehavioral studies in EPM rats withOcimum sanctum and its combination(SM) dose significantly shows more entries (p=0.0003) and time spent in the open arm (p<0.0001) and also increased entries in centre squares in the open field test which reduced anxiety(p<0.0001). Conclusion: On the basis of the result obtained in this study we can suggest that Ocimum sanctum may have protective/therapeutic and neuroprotective effects in seizures.