Background and Objectives: Thyroid disorders are globally prevalent conditions. Maintaining optimal thyroid hormone levels is essential for normal reproductive function. The incidence of thyroid disorders increases with age. Women often experience menstrual cycle-related symptoms and are at a higher risk of developing thyroid dysfunction. This study aimed to compare the thyroid profile among pre and postmenopausal women. Materials & methods: 85 premenopausal women (age >40 years) and 90 postmenopausal women (menopause duration ≤ 5 years) were selected. Thyroid profile assessment was conducted in all subjects in a basal-fasting state, ensuring standardized conditions. Results: The mean levels of thyroid-stimulating hormone (TSH), FT3, and FT4 were higher in the postmenopausal women. Similarly, the mean levels of were slightly higher in the postmenopausal group. However, no statistically significant differences were observed in the thyroid hormone profile between the pre and postmenopausal groups. Conclusion: Although the current study did not yield statistically significant differences, it is important to that further research with a larger sample size in the Indian population is necessary to thoroughly evaluate the association between thyroid profile and premesh and postmenopausal status. Key words: Female, Menopause, Thyroid Hormones, Menstrual Cycle