Introduction: India has an estimated 2.3 million HIV positive persons. Sexual route appears to be the major mode of transmission though injectable drug use is also emerging as an important mode of transmission in some parts of the country3.HSV‑1 & HSV‑2 are common human pathogens which may cause severe illness. This study was aimed to observe the burden of HSV coinfection among the HIV positive cases in Jaipur Rajasthan Materials & Methods: Total 100 HIV Positive patients were enrolled in this study. Samples were collected from the patientsattending at ICTC, from MGMCH Jaipur from period of 2019-22 after taking ethical approval from MGUMST University.All such patients were tested for seroprevalence of HSV1 & HSV2 by Calbiotech HSV1 & HSV2 IgM & IgG detection kits as per the standard protocol. Results: Out Of 100 HIVpatients, 62% were males while 38% were females. The mean age of the HIV positive patients was 31-50 yrs (59%) and the male to female ratio was 1:1.63. Among these HIV Positive cases HSV1 IgM were -27%, HSV1 -IgG 33%, HSV2 -IgM 10% & HSV2-IgG 12%. Conclusion: The seropositivity of HSV was found to be higher in HIV‑positive patients which indicates that the two viruses collaborate in infection of patients. HSV-1 was predominant among males than females and was more evident among patients with reduced CD4 counts. Keywords: HIV, Coinfection HSV, Seroprevalence HSV.