When an LSTV is present, the preferred modality is radiograph (Ferguson radiograph) which helps in identification of the individual lumbar vertebral level by the following observations on lumbosacral radiograph: the largest transverse pedicle diameter and the longest inter-pedicular distance is at L5; the longest transverse process is at L3; the neural foramina gradually increase in size from L1 to L5; the iliac wings are located between L4 and L5. Most often lumbar spine imaging is done in cases of non-traumatic low backache and the MRI is the preferred modality in such cases because of its soft tissue resolution. Totally 224 Indian subjects who underwent lumbar spine MRI with whole spine screening in Radiology department were studied. Most of these subjects were referred for evaluation of backache and radiculopathy. After reviewing 21 patients with prior spine surgery, severe alterations in spine curvature, extremely tortuous aorta and spine deformities were excluded. Among the subjects with normal segmentation majority of them had aortic bifurcation at the level of Upper L4 (33.7%), Mid L4 (28.5%).Among the subjects with sacralization majority of them had aortic bifurcation at the level of L3-L4 (27.8%), Mid L3 (22.2%). Among subjects with Lumbarization majority of them had aortic bifurcation at the level of L4 lower (40%).