Background: To determine the effect of intraperitoneal local anaesthetic instillation on postoperative pain relief following laparoscopic cholecystectomy. Methods: In this randomised controlled study, 64 patients with gallstones were randomly assigned to one of two groups for laparoscopic cholecystectomy. Group A received intraperitoneal irrigation of the diaphragmatic surface and gallbladder fossa with 0.5% bupivacaine, whereas Group B received no intraperitoneal medication. Results: In the bupivacaine group, there were 27 female patients and 5 male patients with an average age of 46.12 years, whereas in the control group, there were 28 female patients and 4 male patients with an average age of 45.36 years. At 8, 16, and 24 hours, the mean VAS scores in the bupivacaine group were 3.875, 2.5625, and 0.75, while they were 6.50, 3.25, and 0.875% in the control group. Females tended to have higher VAS scores. In the first eight- and sixteen-hours following surgery, the control group required more analgesia than the experimental group (87.50% and 56.25 %, respectively). 24 hours after surgery, the need for post-operative analgesia was nearly equivalent in both groups. In the first eight hours after surgery, pain relief was greater in the bupivacaine group, 31.25 versus 12.5%. Conclusion: Intra-peritoneal instillation of Bupivacaine decreases early post-operative VAS scores and pain intensity after 8 and 16 hours, but it has no long-term effect on post-operative pain alleviation and does not reduce the need for analgesics.