Introduction-Breast milk is the first natural food for the infants. Breast milk ismost vital for infant growth and development. Breastfeeding protect mothers inseveral ways from harmful diseases. It develops a strong bond between motherand infants. Exclusive Breastfeeding is an important practice for the infantsgrowth and development. The purpose of the present study was to study the current status of Breast feeding knowledge and attitude among mothers. Methods- It was a cross-sectional study with 190 mothers participated fromUnited Institute of Medical Science, Prayagraj. Subjects detailed demographic profile were noted down. Subjects knowledge and attitude regarding breast feeding was assess on the basis of standardized questionnaire(IIFAS). Result- Subjects demographic data represents that most of the mothers were housewives with primary education. Questionnaire based on knowledge andattitudes reports that mothers have good knowledge regarding the breastfeeding but has a neutral attitude towards breast feeding practice. Conclusion- In the present study mother’s knowledge and attitude regarding breastfeeding was positive, but breastfeeding practice was neutral. Itwasevidentthatthereisagapbetweenknowledgeandpracticeonbreastfeeding.Thusfurthermore schooling regarding importance of breast milk and breastfeeding should behighlightedamong women.