Aim: To assess the effectiveness of PRP in the treatment of anal fistula Method and material: Total100 patients with anal fistula were selected and divided into 2 groups. In patients with Group 1 PRP was administered topically just immediately after fistulous tracts excision(study group) while in group 2PRP therapy was not given after fistulectomy (control group).Patients were evaluated clinically for anal pain, incontinence, discharge, and/or infection at each visit. If the patient became asymptomatic (no pain with a closed external opening), the procedure was regarded successful. After six months, the surgical procedure was deemed unsuccessful if pain, suppuration, or external opening persisted. Results:It was observed that after 10 days in group 1 only one (2%) patients had infection while in control group 6(12%) patients had infection(p=0.05); 4(8%) patients in study groups while in control group 5(10%) patients had pain(p=.72)(table 1).After 3 month no recurrence was seen in study group while the recurrence rate was 12% after 3 month in control group(p<0.05). While after 6 months the recurrence rate was 4% and 2% in study and control group respectively(p>0.05).The time taken by study group was significantly less then taken by control group (p<0.001). Conclusion: In present study PRP showed favorable safety and was seen to be effective in the treatment of anal fistula on combining with the fistulectomy.