Background: Alcoholic liver disease (ALD) is predominant form of chronic liver disease reported up-till now. Gamma glutamyl transferase(GGT) and AST/ALT ratio are routinely used as diagnostic tool in Alcoholic liver disease. However, no single laboratory marker is definitely helpful in detecting alcohol related injuries. There is always a need of combining different markers so as to be sensitive and specific enough to detect alcoholic liver disease. Hence, present study conducted to find role of GGT and AST:ALT ratio in isolation as well as in combination in diagnosing alcoholic liver disease. Method: It is cross sectional study conducted in tertiary care hospital. ALD patients diagnosed using case definition – on the basis of ‘CAGE questionnaire, clinical evaluation and fatty liver on Ultrasonography’ were enrolled and their serum GGT and AST:ALT levels were measured. Result: among 110 enrolled participants, Independently GGT has sensitivity of 96.4% and specificity of 92.7% and that of AST/ALT ratio is 87.3% and 90%. However, it was observed that Sensitivity of tests was highest (100%) when done concordantly and specificity of tests was highest (98.2%) when done discordantly as opposed to GGT and AST/ALT ratio done separately. Conclusion: GGT & AST/ALT used concordantly can be best utilized as screening test, however for definitive diagnosis both tests used discordantly are more useful.