Aim: To Assess the Knowledge and Attitude regarding Immunization among Mothers of Under Five Children in selected Urban and Rural areas of Faridkot, Punjab. Material and Methods: A quantitative research approach and comparative descriptive research design was used. The Sample size was 100 mothers of under five children, selected by non probability purposive sampling technique. The data were collected by using structured interview schedule with the help of self structured knowledge questionnaire and attitude scale (5 points likert scale). Descriptive and inferential statistics were used to analyze the data. Results: The study revealed that in urban area was 72% had adequate knowledge and 28% had inadequate knowledge regarding immunization and in rural area 68% mothers had adequate knowledge and 32% had inadequate knowledge regarding immunization. In urban area 76% had highly positive attitude and 24% had low positive attitude regarding immunization and in rural area 86% had highly positive attitude and 14% had low SD for knowledge score of mothers±positive attitude regarding immunization. The mean 5.00 for urban and rural area's mothers respectively. So± 5.60 and 18.16 ±were 20.34 SD were slightly high in urban mothers than rural mothers. The results were±mean SD for attitude score of mothers±found statistically significant at 0.05 level. The mean 11.00 for urban and rural area's mothers respectively. So± 10.89 and 67.56 ±were 61.76 SD were slightly high in rural mothers than urban mothers. The results were±mean found statistically significant at 0.05 level. In urban area there was significant relationship between knowledge score with Education of Mother, Monthly Income of family, Number of children, Source of information and in rural area significant relationship with occupation of Mother and religion of family. In urban area there was significant relationship between attitude score with Monthly Income of family and Source of information and in rural area there was significant relationship between attitude score with Age of mothers and source of information. Conclusion: From the findings of the study following conclusion were drawn that the level of knowledge mothers of urban area were higher than the mothers of rural area and the attitude of mothers of rural area were highly positive than the mothers of urban area.