Background:Dengue infection is the most prevalent arthropod-borne viral disease in subtropical and tropical regions of the world. The present study was conducted to assess the effectiveness of antibodies in diagnosing cases of dengue fever. Materials & Methods: This study was conducted on 142 patients. For detection of NS1 antigen, IgG and IgM antibody rapid immune chromatography method was used. Results:Out of 142 patients, males were 82 and females were 60. Common clinical features were headache in 76, vomiting in 89, abdominal pain in 94, myalgia in 52, hepatomegaly in 48 and bleeding in 36 cases. The difference was significant (P<0.05). Among serum samples, 90% found positive for NS1, IgG (4%), IgM (2%). 1.3% for both NS1 and IgG, 1.2% for NS1 and IgM and0.50% for NS1, IgM and IgG. The difference was significant (P<0.05). Conclusion: Dengue fever is common nowadays. It was found that NS1, IgG and IgM are diagnostic marker for dengue fever.