Background:Bleeding time (BT), clotting time (CT), and blood grouping constitute standard procedures in hematology experiments within physiology labs. In the medical realm, these tests are pivotal in conditions such as thrombosis and epistaxis prior to surgical interventions. Achieving effective hemostasis holds paramount significance for both surgeons and anesthetists. While numerous investigations have explored the correlation between blood groups and various diseases, limited research has delved into the connection between blood groups and BT, CT. The study's primary objective is to evaluate the interrelationship between BT and CT and different ABO blood groups.Methods:Conducted within the Department of Physiology, this investigation involved 400 students as participants. The determination of blood groups was executed through the mixing of blood samples with antisera A, B, and antisera Anti D. Results:The study outcomes revealed that blood group O (37%) exhibited higher prevalence, followed by blood groups B (30.5%), A (23.5%), and AB (9%). Notably, bleeding time was observed to be prolonged (>3 minutes) in the AB blood group (37.5%), with group O (25%), A (25%), and B (12.5%) following suit. This difference was found to be statistically significant (p=0.03), emphasizing a noteworthy association between blood groups and prolonged bleeding time.Conclusion:The findings from our study highlight that blood group O emerged as the most prevalent, whereas AB stood as the least common. Notably, bleeding time (BT) was prolonged in the AB blood group, contrasting with clotting time (CT) being extended in the O blood group. Furthermore, our results indicate that clotting time was more prolonged in females compared to males.