Background: Vitamin D is basically available from two sources: exposure to UV light of sun and dietary sources. Epidemiologic studies have shown that the prevalence of multiple sclerosis is more at higher latitudes and tends to be higher in areas where there is low exposure to ultraviolet light. The present study was aimed to determine the association between Vitamin D levels and multiple sclerosis relapse. Materials and Methods: The study was conducted in the department of Neurology, B. J. Medical College, Ahmedabad, Gujarat(India) for 1 year duration. It enrolled all the subjects with more than 6 months’ duration of multiple sclerosis and that was confirmed by Mcdonald’s criteria. Clinical characteristics of all the MS subjects were obtained from the hospital records. The EDSS score of all the subjects was obtained at the time of serum collection. The basic features of all the patients like age, Gender were also noted. All the data obtained was arranged in a tabulated form and analyzed using SPSS software. Data was arranged as percentage. The levels of Vitamin D were noted in Nmol/L. Results: There were 21 males and 29 females in the study. The mean age of the study population was 45.87+/-11.29 years. The mean EDSS study of the sample was 4.5 +/-2.1. There were 60% subjects of multiple sclerosis with suboptimal levels of vitamin D and 32% subjects had normal levels of vitamin D. Conclusion: The therapeutic values of vitamin D are yet to be explored. In our study, the subjects with multiple sclerosis had lower levels of vitamin D.