Background: The human immunodeficiency virus (HIV) is the main cause of acquired immunodeficiency syndrome (AIDS), which has been the biggest threat to humanity for the past thirty years. According to a UNAIDS (2010) Global Report, 33.3 million people are HIV positive globally. The purpose of this study was to determine the prevalence and risk factors of HIV infection by a retrospective analysis. Materials and Procedures: A six-month study was conducted in an ICTC, index medical college, and hospital in Indore from January to June of 2023. Result: HIV prevalence in this study was determined to be 11% (77/700). 77 of the 700 clients in total had positive HIV tests. Conclusion: The high risk groups that have been identified should get targeted interventions as well as intensive information education and communication (IEC) in various settings.