Aim:The purpose of this research was to determine the minimum threshold levels of plasma and urinary lactate that should be used to diagnose EOS. Material and Method :The study comprised of ninety neonates at risk for early-onset sepsis. The following tests were performed: sepsis screening, blood culture, plasma lactate within two hours, and urinary lactate in the initial urine sample. At 24 ± 2 hours, CRP, plasma, and urinary lactate measurements were repeated. Results: In the sepsis group, the median urinary lactate levels were 0.5 mMol/L and 0.45 mMol/L at the first passed sample, respectively, while in the non-sepsis group, they were 0.37 mMol/L and 0.43 mMol/L at 24 hours. Neither plasma nor urinary lactate could be utilized to diagnose sepsis with an early onset. In contrast, urinary lactate demonstrated utility as a diagnostic indicator for sepsis accompanied by mortality and shock. Conclusion: Our research concludes that plasma and urinary lactate do not predict EOS, urinary lactate can predict shock and mortality in infants with EOS at 24 hours of life