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Volume 12 Issue 2 ( April- June) 2023

Original Articles

Usefulness of rapid modified leishman stain in peripheral blood smear: A cross sectional study
Dr. Rajini T, Dr. Mallika B, Dr. Amita K, Dr. Sanjay M, Dr. Ullas Mahesh

Background: Diagnosis of few disease and during emergencies peripheral blood smear is routinely done and is also the basic tool for the diagnosis of different hematological conditions. Romanowsky stains are available for staining smear in which, Leishman stain is most commonly used in laboratories worldwide. Various studies have been done to reduce the staining duration. The present study was conducted with aim to know the usefulness of rapid Leishman stain, by calculating the Quality Index (QI) of the stained smears and comparing the scores with that of routine stained smears. Methods: Study was done in the hematology department from November 2021 upto January 2022 Modified rapid Leishman stain (MLS) was prepared in which phenol was added to the Leishman stain. 101 cases of peripheral blood smears were taken, and four sets of the smears were prepared from each sample-one for routine staining and other three for rapid modified leishman stain on day 1, 5 and 10 after preparing the stain. After staining, smears were scored based on overall staining, cytoplasmic staining, nuclear morphology, red cell staining and platelet staining. Quality index was calculated by dividing the score obtained by maximum score possible. Result: The overall staining, cytoplasmic staining, nuclear morphology, red cell staining and platelet staining were better on day 10 after preparation of MLS when compared to day 1 and day 5. The QI of normal leishman stained smear was 0.95, whilst that of MLS stained smear was 0. On day 1, 0.73 on day 5 and 0.89 on day 10 of preparing stain. Conclusion: Rapid Lesihman stain can serve as an alterantive to Leishman stain in emergency situation.

 
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