Abstract—Hydrogen is a valuable gas as a clean energy source; biological processes utilized for hydrogen gas production are bio-photolysis of water by algae, dark and photo-fermentation of organic materials, by bacteria. Dark fermentation process is a new approach, but expensive due to raw material cost. Carbohydrate rich, nitrogen deficient solid wastes including dairy waste can be used for hydrogen production by using suitable bio-process technologies. Mixed microbial consortia of Enterobacter cloaceae and Clostridium pasteurinum were used for the biohydrogen production. An anaerobic continuous stirred tank reactor with working volume of 12.8L was constructed and operated for 20 days. The temperature of the CSTR was regulated at 37 ¡ãC. The pH was controlled at 6.0. A continuous hydrogen gas production was achieved and analysed using gas chromatography.