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An In Vitro Callus Induction and Isolation, Identification, Virtual Screening and Docking of Drug from Convolvulus alsinoides Linn Against Aging Diseases

D S V G K Kaladhar
Department of Biochemistry/Bioinformatics, GIS, GITAM University, Visakhapatnam, India
Abstract—An in vitro callus induction and in silico analysis in Convolvulus alsinoides, an ancient Indian traditional medicinal plant can provide evidence in soft callus formation, isolation and virtual screening of medicinal compounds. In this experiment, an investigation was done to find out a suitable and standard protocol for callus induction of an Indian traditional medicinal plant, Convolvulus alsinoides Linn. Higher concentrations of 2, 4-D (≥3mg/l) was more effective for callus induction on terminal buds and flower explants. The experimental results of calli were shown as brownish white, friable and ascribed meristematic nature. Leaving the calli in the medium turned brown and dried within 4 months. Isolation of medicinal compounds from callus is easier as the callus is soft and available throughout the year in lab conditions. Kaempferol, an isolate from callus of C. alsinoides predicted good activity in decreasing fat levels, and also have good effect against Alzheimer and Parkinson diseases. Kaempferol predicted least activity against Type 2 Diabetes Mellitus. Various compounds can be isolated from callus cultures and have medicinal usage. The present study can be helpful in callus induction and virtual screening of products from plant species such as Convolvulus alsinoides. 

Index Terms—convolvulus alsinoides Linn, callus induction, virtual screening and docking

Cite: D S V G K Kaladhar, "An In Vitro Callus Induction and Isolation, Identification, Virtual Screening and Docking of Drug from Convolvulus alsinoides Linn Against Aging Diseases," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 1, No. 3, pp. 93-103, July 2012.
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