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Isolation, Identification and Screening of Pectinolytic Fungi from Different Soil Samples of Chittoor District

P Lakshminarasimha Reddy and A Sreeramulu
Department of Botany, S V University, Tirupati 517502, AP, India
Abstract—The aim of this present study was isolation and screening of pectinase producing fungi from different agricultural and non agricultural soils in chittoor district, Andhra Pradesh, South India. Total 44 fungal strains were isolated from these soils and only 4 strains were positive in pectinolytic activity. The extracellular pectinase was partially purified by ammonium sulphate precipitation and dialysis. These 4 strains were identified as Aspergillus niger, A. flavus, A. japonicus, and Chaetomium globosum and they produced very high levels pectinase by submerged fermentation. Maximum enzyme production was obtained in the medium containing citrus pectin. The effect of different temperature shows the optimum temperature for enzyme production was 30°C. 

Index Terms—aspergillus sp. pectinase, submerged fermentation

Cite: P Lakshminarasimha Reddy and A Sreeramulu, "Isolation, Identification and Screening of Pectinolytic Fungi from Different Soil Samples of Chittoor District," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 1, No. 3, pp. 186-193, July 2012.
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