Abstract—Shiga toxin producing E. coli (STEC) is an emerging pathogen. These STEC strains are grouped as O157 and non O157 group. These non O157 include several serogroups. These strains also cause outbreaks similar to that of O157 E. coli. Antibiotic resistance is increasing among these STEC and usage of antibiotics for treatment of such strains result in induction of toxin production. Hence, phytochemicals can be used as an alternative to antibiotics. Punica granatum peels are rich in polyphenols, tannins and other secondary metabolites. The objective of this study is to analyse the bioactive phyto components of Punica granatum peel extracts against Shiga toxin producing E. coli. The methanol extract of P. granatum peel showed high antibacterial activity against STEC. HPLC analysis revealed Punicalagin ( 㰰〴㐾 ) and ( 㰰〴㔾 ). Ellagic acid was also present in the extract. GC-MS analysis revealed the presence of 36 compounds, some of them were not reported previously.

Index Terms— Phyto chemical Analysis, GC-MS analysis, Ethyl acetate extract, Punica granatum , Shiga toxin producing E. coli

Cite: Lali Growther, Sukirtha K., Savitha N. and Niren Andrew S., "Antibacterial Activity of Punica granatum Peel Extracts against Shiga Toxin Producing E. coli," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 1, No. 4, pp.164-172, October 2012.