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Collection and Screening of Basidiomycetes for Better Lignin Degraders

D. Seshikala and M. A. Singara Charya
Kakatiya University College of Engineering, Warangal, Andhra Pradesh, India.
Abstract—Considering the potentialities of white rot basidiomycetes in biobleaching process, 37 white rot fungi were collected from different forest areas of Andhra Pradesh, India. All of them were screened for lignolytic enzyme production. Out of them 25 different organisms were with lignolytic capacity. Then they were quantitatively and qualitatively analyzed for Laccase, LiP and MnP enzymes. Among the studied organisms Stereum ostrea (Laccase 40.02U/L ,MnP 51.59U/L, LiP 11.87U/L), Tremella frondosa (Laccase 35.07U/L, MnP 29.12U/L, LiP 5.95U/L) Tremates versicolour (MnP and LiP production i.e 56.13 U/L, LiP 23.26 U/L) could show maximum enzyme production. All the 25 organisms could produce Laccase but few failed to produce MnP and LiP. The organisms which produced both enzymes were grown in the liquid cultures. That culture filtrate was used for qualitative (SDS PAGE)and quantitative (enzyme assay) analysis.

Index Terms—Basidiomycetes, White rot fungi, Lignolytic enzymes, Laccase, MnP.

Cite: D. Seshikala and M. A. Singara Charya, "Collection and Screening of Basidiomycetes for Better Lignin Degraders," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 1, No. 4, pp.203-211, October 2012.
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