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Genetic Characterization of Chlamydia Pneumoniae Using Restriction Endonuclease Enzyme (Xbal) In Deltans Nigeria

Otoikhian C. S. O., Ekakitie A. O., and Okoror L. E.
Novena University, College of Natural and Applied Sciences, Department of Biological Sciences, PMB 2, Kwale-ogume, Delta State, Nigeria.
Abstract—Restriction endonuclease enzyme (XbaI) is a cutting enzyme and as such was used to characterize the organism. Of the 124 samples collected from different locations in Mid-Western Nigeria, 95(76.61%) were positive while 29(23.38%) were negative for Chlamydia pneumoniae by DNA isolation. The enzyme cut the DNA of Chlamydia pneumoniae at several points as visualized on agarose gel placed on ultra-violet illuminator. Only 3 DNA could not be cut by this enzyme. This shows strain variation.

Index Terms—DNA Isolation, Polymerase chain reaction, Chlamydia trachomatis, Chlamydia pneumoniae

Cite: Otoikhian C. S. O., Ekakitie A. O., and Okoror L. E., "Genetic Characterization of Chlamydia Pneumoniae Using Restriction Endonuclease Enzyme (Xbal) In Deltans Nigeria," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 1, No. 4, pp.222-231, October 2012.
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