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Antioxidant and Antibacterial Activity of Leaf and Bark Extracts of Seabuckthorn (Hippophae salicifolia D. Don) of North East India

Mousmi Saikia and PJ Handique
Department of Biotechnology, Gauhati University, Guwahati, 781014, India.
Abstract—The present study was conducted to evaluate the antioxidant and antibacterial activities of different solvent extracts of leaf and bark of Seabuckthorn ( Hippophae salicifolia ) species found in North East India.Antioxidant activity was measured using total phenolics content, reducing power and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Methanolic extract of both leaf and bark samples showed highest antioxidant activities compared to the extracts in other solvents. Antibacterial activity was tested against six pathogenic strains by agar diffusion and broth macrodilution methods. The extracts were found to exert low to moderate antibacterial activity compared to a standard antibacterial agent. Both antioxidant and antibacterial activities were found to be higher in methanolic extract. Strong correlation ( P \< 0.05) was observed between total phenolic content versus antioxidant and antibacterial activity from the extracts under study.

Index Terms—The present study was conducted to evaluate the antioxidant and antibacterial activities of different solvent extracts of leaf and bark of Seabuckthorn ( Hippophae salicifolia ) species found in North East India.Antioxidant activity was measured using total phenolics content, reducing power and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Methanolic extract of both leaf and bark samples showed highest antioxidant activities compared to the extracts in other solvents. Antibacterial activity was tested against six pathogenic strains by agar diffusion and broth macrodilution methods. The extracts were found to exert low to moderate antibacterial activity compared to a standard antibacterial agent. Both antioxidant and antibacterial activities were found to be higher in methanolic extract. Strong correlation ( P \< 0.05) was observed between total phenolic content versus antioxidant and antibacterial activity from the extracts under study.

Cite: Mousmi Saikia and PJ Handique, "Antioxidant and Antibacterial Activity of Leaf and Bark Extracts of Seabuckthorn (Hippophae salicifolia D. Don) of North East India," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 1, pp. 80-91, January 2013.
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