1. How to submit my research paper? What’s the process of publication of my paper?
The journal receives submitted manuscripts via email only. Please submit your research paper in .doc or.pdf format to the submission email: ijlbpr@ejournal.net.
You’ll be given a paper number if your submission is successful. Your paper then will undergo peer review process, which may take approximately one and a half months under normal circumstances, three tops.
After blind peer review, you will receive the notification letter with the review result of your paper...
2. Can I submit an abstract?
The journal publishes full research papers. So only full paper submission should be [Read More]

Biochemical and Molecular Detection of Biosurfactant Producing Bacteria from Soil

Tambekar D. H. and P. V. Gadakh
Department of Microbiology, SGB Amravati University, Amravati 444602.
Abstract—The use of biosurfactant is a promising alternative over the chemical surfactant as they are better biodegradable and do not pollute the environment. Most of the biosurfactant producing microorganisms have been isolated from the hydrophobic environment such as oily waste, sludge etc. in this study, petroleum contaminated soil was collected and enriched on oil containing media for isolation of biosurfactant producing bacteria. From these soil samples 18 different bacterial species were isolated and screened for biosurfactant production by surface tension measurement, drop collapse test, oil displacement test, emulsification index and a-haemolysis. Further all these isolates were identified on the basis of biochemical and 16S rRNA sequencing. Result of biochemical and phylogenic characterization showed that the bacterial were grouped into seven genus and species are Pseudomonas aeruginisa, Aneurinibacillus miugulanus, Bacillus circulans, Ochrobactrum oryzae, Ochrobactrum pseudintermedium, Ochrobactrum intermedium, Achromobacter insolitus, Nocardia farcinica and Stenotrophomonas maltophilia. The result of the phylogenic tree provided a roadmap to the future research, especially in isolation and identification of new biosurfactant-producing strains as well as the produced biosurfactant.

Index Terms—The use of biosurfactant is a promising alternative over the chemical surfactant as they are better biodegradable and do not pollute the environment. Most of the biosurfactant producing microorganisms have been isolated from the hydrophobic environment such as oily waste, sludge etc. in this study, petroleum contaminated soil was collected and enriched on oil containing media for isolation of biosurfactant producing bacteria. From these soil samples 18 different bacterial species were isolated and screened for biosurfactant production by surface tension measurement, drop collapse test, oil displacement test, emulsification index and a-haemolysis. Further all these isolates were identified on the basis of biochemical and 16S rRNA sequencing. Result of biochemical and phylogenic characterization showed that the bacterial were grouped into seven genus and species are Pseudomonas aeruginisa, Aneurinibacillus miugulanus, Bacillus circulans, Ochrobactrum oryzae, Ochrobactrum pseudintermedium, Ochrobactrum intermedium, Achromobacter insolitus, Nocardia farcinica and Stenotrophomonas maltophilia. The result of the phylogenic tree provided a roadmap to the future research, especially in isolation and identification of new biosurfactant-producing strains as well as the produced biosurfactant.

Cite: Tambekar D. H. and P. V. Gadakh, "Biochemical and Molecular Detection of Biosurfactant Producing Bacteria from Soil," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 1, pp. 204-211, January 2013.
Copyright © 2012-2015 International Journal of Life Sciences Biotechnology and Pharma Research, All Rights Reserved