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Influence of Various Factors on Erythropoietin Production Expressed in Chinese Hamster Ovary Cells

Indrapal Reddy Addela1, Ravinder Rudravaram 2, Lakshmi Narasu M.3, and Ramesh B.3
1. Sudershan Biotech, Hyderabad, India.
2. Sowbhagya Biotech (P) Limited, Plot # 79, Phase - II, IDACherlapally, Nacharam, Hyderabad, Andhra Pradesh 500051, India.
3. Department of Biotechnology, JNTUH, Hyderabad, India.
Abstract—The erythropoietin was expressed in Chinese Hamster Ovary (CHO) cells and culture techniques were developed, purification process was developed. Different culture conditions were tried to evaluate the influence of various factors on erythropoietin production expressed in Chinese Hamster Ovary Cells. The productivity was checked at different harvests and found that the EPO expression was stable upto 10 harvest, sialidase activity was checked, effect of addition of sodium butyrate was studied on the productivity of Erythropoietin (EPO).

Index Terms—Chinese Hamster Ovary cells, Erythropoietin, Glycopeptides, Sialic acids

Cite: Indrapal Reddy Addela, Ravinder Rudravaram, Lakshmi Narasu M., and Ramesh B., "Influence of Various Factors on Erythropoietin Production Expressed in Chinese Hamster Ovary Cells," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 1, pp. 212-216, January 2013.
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