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Study of Viability Period of Culture Suspension of Salmonella enterica

Kundan Kumar and Sandhya Jha
University Department of Bio-Technology, L. N. Mithila University, Darbhanga-846004, Bihar.
Abstract—Salmonella enterica ATCC 14028is an important strain of pharmaceutical and biotechnological industries. The present study indicates the viability of culture suspension of Salmonella enterica strain ATCC 14028 at 2 to 8¡ãC up to 360 days in 0.9% w/v NaCl. The culture suspension containing 10000 cfu/ml used in the study was stored at 2 to 8¡ãC for 360 days in 0.9% w/v NaCl. Using 10 㰰〵〾 l of above culture suspension, the viable count was made by the pour plate technique using Soyabean Casein Digest Agar medium in fixed interval of time during the 360 days of storage period. During the storage period, population of Salmonella enterica ATCC 14028decreased from 10000 cfu/ml to 9900 cfu/ml during the first 30 days, whereas the population decreased to 100 cfu/ml in 360 days. Findings emanate from the study indicates that 30 days storage period of Salmonella enterica ATCC 14028at 2 to 8¡ãC in 0.9% w/v NaCl is suitable for laboratories testing purposes on account of fact that in 30 days storage period, population of Salmonella enterica ATCC 14028 decreased from 10000 cfu/ml to 9900 cfu/ml, which is very low.

Index Terms—Microbial limit test, Non sterile products, Sterility test, Sterile products, Viability.

Cite: Kundan Kumar and Sandhya Jha, "Study of Viability Period of Culture Suspension of Salmonella enterica," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 1, pp. 263-266, January 2013.
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