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Evaluation of Salt-Out Method for the Isolation of DNA from Whole Blood: A Pathological Approach of DNA Based Diagnosis

Radheshyam Maurya1,2, Brijesh Kumar 1, and Shyam Sundar 1
1. Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India.
2. Department of Animal Sciences, University of Hyderabad, Hyderabad 500046, India.
Abstract— A number of methods have been described for the isolation of genomic DNA from whole blood. However, these described methods are either unreliable or too expensive to be used routinely in small diagnostic laboratories of developing countries. We have evaluated the slightly modified salt-out method for DNA isolation so that it can be applied on whole blood samples using a three step procedure: (1) lysis of RBC; (2) removal of protein; and (3) precipitation of DNA. We compared the yield of DNA per mL of blood using our modified salt-out DNA extraction method, (40.8±4.3µg) with Phenol-chloroform extraction (38.5 ±7.3 µ g) or QIAamp DNA Mini kits (35. 3± 5.4 µ g). The described method allows simple, fast and cost efficient DNA extraction with qualitative parameters maintained and comparable to those of other extraction method described.

Index Terms— RBC, Red blood cells, Ethylene diaminetetraacetic acid (EDTA), White blood cells (WBC), Polymerase chain reaction (PCR)

Cite: Radheshyam Maurya, Brijesh Kumar, and Shyam Sundar, "Evaluation of Salt-Out Method for the Isolation of DNA from Whole Blood: A Pathological Approach of DNA Based Diagnosis," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 2, pp. 53-57, April 2013.
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