1. How to submit my research paper? What’s the process of publication of my paper?
The journal receives submitted manuscripts via email only. Please submit your research paper in .doc or.pdf format to the submission email: ijlbpr@ejournal.net.
You’ll be given a paper number if your submission is successful. Your paper then will undergo peer review process, which may take approximately one and a half months under normal circumstances, three tops.
After blind peer review, you will receive the notification letter with the review result of your paper...
2. Can I submit an abstract?
The journal publishes full research papers. So only full paper submission should be [Read More]

Effect of Inhibitors on the Acetylcholinesterase Enzyme and Live Worms of Setaria Cervi

Shravan K Singh1, Sunita Saxena1, Shakir Ali2, Deep C Kaushal3, and Nuzhat A Kaushal1
1. Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow-226001, India
2. Department of Biochemistry, Jamia Hamdard, Delhi, India
3. Amity University Uttar Pradesh, Lucknow Campus, Lucknow-226010, India
Abstract—Filariasis is a major health problem, affecting millions of people in tropical and subtropical regions of the world. Targeting the parasite specific enzyme is not only rational but essential for developing effective control measure against filariasis. In our earlier studies we have identified two isozymic forms of acetylcholinesterase (AchE) from bovine filarial parasites (Setaria cervi) different from the host AchE. In the present study, we have studied the effect of enzyme inhibitors (BW284c51, Edrophonium and Eserine) and antifilarial drugs (Diethylcarbemazine and Ivermectin) on parasite acetylcholinesterase and on live adult worms. Out of different inhibitors, only inhibitor BW284c51, strongly inhibited (98%) the enzyme activity of S. cervi acetylcholinesterase with an IC50 value of 26.87 μM at 0.50 mM substrate concentration. Lineweaver–Burk transformation of the inhibition kinetics data demonstrated that it was a competitive inhibitor of filarial acetylcholinesterase and Ki value was 9.3 μM. However, in vitro incubation of live S. cervi adult worm with 100 μM concentration of BW284c51 resulted in only 40% inhibition of parasite acetylcholinesterase and 50% reduction of worm motility. These findings suggest that BW284c51 is a potent competitive inhibitor of filarial acetylcholinesterase and AchE can be a potential target for rational screening of antifilarial compounds. 

Index Terms—setaria cervi, acetycholinesterase, BW284c51, ISO-OMPA, diethylcarbemazine, ivermectin

Cite: Shravan K Singh, Sunita Saxena, Shakir Ali, Deep C Kaushal, and Nuzhat A Kaushal, "Effect of Inhibitors on the Acetylcholinesterase Enzyme and Live Worms of Setaria Cervi," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 3, pp. 144-151, July 2013.
Copyright © 2012-2015 International Journal of Life Sciences Biotechnology and Pharma Research, All Rights Reserved