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Antimicrobial Activity of Xylopia Sethiopica (Uda) on Escherichia coli and Staphylococcus aureus Isolates From Gastroenteritic patients

Ikeyi Adachukwu P1, Ogbonna Ann O1, Ibekwe Rita O1, and Ugwu Okechukwu P C2
1. Department of Science Laboratory Technology Institute of Management and Technology (IMT) Enugu, Nigeria
2. Department of Biochemistry University of Nigeria Nsukka, Enugu State, Nigeria
Abstract—Xylopia aethiopica (Uda) is a slim, tall, evergreen aromatic medicinal plant of West Africa which produces a variety of complex chemical compounds. The dried fruits showed various degrees of activity against gram positive bacteria Staphylococcus aureus and gram negative bacterial Escherichia coli isolated from gastroenteritic patients. Anti-microbial screening test of (Uda) extract was carried out on the isolated organism using Agar diffusion and disc diffusion methods in various (Uda) extracts (aqueous, hot and ethanol extract) at varied concentrations. However, the results showed different zones of inhibition and Minimum Inhibitory Concentration (MIC). Aqueous extract of X. aethopica had MIC of 50µg/ml, hot extract had MIC of 25µg/ml while ethanol extract had MIC of 50µg/ml and 75µg/ml on the isolated organism. The findings suggest that the anti-microbial activity of X. aethiopica extract resides in their aqueous and hot fractions and also the results indicated that volatile compound (ethanol) should not be used in plant extraction as a result of the escape of active component of the plant extract. 

Index Terms—xylopia aethiopica, uda, escherichia coli, staphylococcus aureus, gastroenteritic patients

Cite: Ikeyi Adachukwu P, Ogbonna Ann O, Ibekwe Rita O, and Ugwu Okechukwu P C, "Antimicrobial Activity of Xylopia Sethiopica (Uda) on Escherichia coli and Staphylococcus aureus Isolates From Gastroenteritic patients," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 3, pp. 330-338, July 2013.
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