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Isolation and Characterization of Nitrogen-Fixing Bacteria in the Soil

Onyeze Rosemary C, Onah Gloria T, and Igbonekwu Cecilia C
Department of Science Laboratory Technology, Institute of Management and Technology (IMT), Enugu, Nigeria
Abstract—Four soil samples were collected from different sources, two of these soil samples were from Thinkers corner land and Emene beans farm land while the remaining two were from maize (Zea mays) and legume farm lands, Ugbochime Community all in Enugu State, Nigeria. The nitrogen-fixing media used were Azotobacter medium, Azospirillum medium and Clostridium medium. The pH of these media were adjusted to 7.4, 6.8 and 5.4, respectively. The isolates were subjected to various biochemical tests for differentiation. The bacteria isolated were Azospirillum, Azotobacter and Clostridium species. Azospirillum formed colonies were milky, slimy, circular and raised while Clostridium spp. produced gas bubbles and rancid odor. 

Index Terms—azospirillum, azotobacter, clostridium, isolates, colonies, soil samples

Cite: Onyeze Rosemary C, Onah Gloria T, and Igbonekwu Cecilia C, "Isolation and Characterization of Nitrogen-Fixing Bacteria in the Soil," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 3, pp. 438-445, July 2013.
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