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Bacteriological Analysis of Pipe-Borne Water and Well Water within Enugu East, Enugu State Nigeria

Onyeze Rosemary C, Onah Gloria T, and Eluke Obinne C
Department of Science Laboratory Technology, Institute of Management and Technology (IMT), Enugu, Nigeria
Abstract—The bacteriological examination of water sourced within Enugu East was carried out to determine the bacterial load in the water samples and to determine the organisms that contaminate it. The water samples were pipe-borne water and well water. They were collected within Enugu East in sterile containers. Using pour plate plating technique 0.1ml of water sample was pipetted into petri dishes and already prepared agar (Nutrient agar and MacConkey agar) were poured into the Per- dishes, swirled for a homogeneous mixture, allowed to solidify and incubated at 37oC for 24 hrs. Presumption coliform counts were carried out by using double strength lactose and single strength lactose broth in test tubes and these were incubated at 37°C for 24°hrs. The result showed high counts of microorganism in well water while pipe-borne water had low counts. The bacterial isolated are Citrobacter spp., Escherichia coli and Klebsiella Spp. These showed that well water contained contaminants and there should be need for adequate treatment of water to avoid water borne diseases such as Cholera, Typhoid Fever, Dysentery and Escherichia coli infection. 

Index Terms—citrobacter spp., escherichia coli, klebsiella spp., bacteriological analysis, coliform counts

Cite: Onyeze Rosemary C, Onah Gloria T, and Eluke Obinne C, "Bacteriological Analysis of Pipe-Borne Water and Well Water within Enugu East, Enugu State Nigeria," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 3, pp. 446-452, July 2013.
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