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Antimicrobial Sensitivity of Aloe vera Extracts on Staphylococcus and Pseudomonas Species Isolated from Wound Sepsis

Onyeze Rosemary C1, Udeh Sylvester M C2, and Mazi Rita O1
1. Department of Science Laboratory Technology, Institute of Management and Technology (IMT), Enugu, Nigeria
2. Department of Biochemistry University of Nigeria, Nsukka, Enugu State Nigeria
Abstract—The antimicrobial sensitivity of Aloe-vera extracts on clinical isolated Staphylococcus and Pseudomonas species were carried out using the Agar gel and Disc diffusion methods. The solvent of extraction were water (cold and hot) and ethanol. Based on this analysis the effects of Aloe-vera extracts were dose dependent. Both water and ethanol extracts were evaluated using varying concentrations of 10 ml/mg, 5 ml/mg, 2.5 ml/mg, 1.25 ml/mg and 0.313 ml/mg. The effects of hot water extracts were high on Staphylococcus spp. at 10 ml/mg (5 mm) and on Pseudomonas spp. (2 mm) at 10 ml/mg. Pseudomonas spp. in cold water had the highest effect at lowest concentration of 0.313 ml/mg (4.5 mm). The effect of ethanol extract was high at lowest concentration of 0.313 ml/mg (4.5 mm) and the minimum inhibitory concentration of water and ethanol extracts was (0.313 ml). 

Index Terms—antimicrobial sensitivity, aloe-vera, pseudomonas spp., staphylococcus spp., agar gel, disc diffusion methods

Cite: Onyeze Rosemary C, Udeh Sylvester M C, and Mazi Rita O, "Antimicrobial Sensitivity of Aloe vera Extracts on Staphylococcus and Pseudomonas Species Isolated from Wound Sepsis," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 3, pp. 453-458, July 2013.
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