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Isolation and Screening of Actinomycetes from Marine Sediments for their Potential to Produce Antimicrobials

Gunasekaran Mohanraj and Thangavel Sekar
Post Graduate and Research Department of Botany, Pachaiyappa's College,Chennai, Tamil Nadu, India
Abstract—Actinomycetes are known to produce potential secondary metabolites which are having biological activity. Studies were conducted to isolate and identify the biologically potential marine actinomycetes and also these studies confirm that the isolates have enormous potential for the production of antimicrobial substances. Marine sediments were collected from 28 points in the Bay of Bengal and totally 52 actinomycetes were isolated using different isolation media. All the above isolates were characterized and identified by microscopical and macroscopical observations. Identification of the isolates revealed that all isolates belong to the genus Streptomyces. The isolated marine actinomycetes were screened for their antimicrobial activity against the human bacterial pathogens Salmonella typhi, Escherichia coli, Bacillus cereus, Staphylococcus aureus and Klebsiella pneumonia and fungal phytopathogens Rhizoctonia solani, Macrophomina phaseolina, Fusarium udum and Fusarium oxysporum f. sp. lycopersici. Among 52 isolates, 43 showed antibacterial activity and 20 isolates showed antifungal activity. The marine isolate Streptomyces sp. LCJ85 was found to be more efficient in the production of secondary metabolites. Also further attention has been paid to study their antimicrobial properties and their other biologically useful properties. 

Index Terms—marine actinomycetes, streptomyces, antibacterial, antifungal, starch casein agar

Cite: Gunasekaran Mohanraj and Thangavel Sekar, "Isolation and Screening of Actinomycetes from Marine Sediments for their Potential to Produce Antimicrobials," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 3, pp. 115-126, July 2013.
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