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Assessment of Antioxidative Potential of Acacia nilotica (L.) Willd Ex Del. Via In Vitro Models

Jyoti Mahajan and Saroj Arora
Department of Botanical & Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India
Abstract—The present study envisages the antioxidant activity of different fractions of leaves of Acacia nilotica Willd. Ex. Del. The leaves of Acacia nilotica after drying and grinding were serially extracted with solvents of increasing polarities to obtain the hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The total phenolic and flavonoid content of the fractions was determined. The antioxidant capacity was done by using Total Antioxidant Capacity (TAC), Cupric ion Reducing Antioxidant Capacity (CUPRAC) and lipid peroxidation assays. It was found that the fractions exhibited the radical scavenging activity in a dose dependent manner. The maximum total phenolic and total flavonoid content was found in ethyl acetate fraction 93.3 GAE mg/g and 61.19 RE mg/g. A maximum inhibition of 93.25 ascorbic acid equivalents (mg/g) of ethyl acetate fraction followed by chloroform was observed in total antioxidant capacity assay. In CUPRAC assay, the ethyl acetate fraction was found to have maximum reduction potential of 99.06% at 200 µg/ml of concentration whereas in lipid peroxidation assay the hexane fraction exhibited the maximum inhibition (86.85%). 

Index Terms—acacia nilotica, antioxidant assays, CUPRAC, lipid peroxidation

Cite: Jyoti Mahajan and Saroj Arora, "Assessment of Antioxidative Potential of Acacia nilotica (L.) Willd Ex Del. Via In Vitro Models," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 2, No. 4, pp. 42-51, October 2013.
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