1. How to submit my research paper? What’s the process of publication of my paper?
The journal receives submitted manuscripts via email only. Please submit your research paper in .doc or.pdf format to the submission email: ijlbpr@ejournal.net.
You’ll be given a paper number if your submission is successful. Your paper then will undergo peer review process, which may take approximately one and a half months under normal circumstances, three tops.
After blind peer review, you will receive the notification letter with the review result of your paper...
2. Can I submit an abstract?
The journal publishes full research papers. So only full paper submission should be [Read More]

Study of Genomic Fingerprints Profile of Indian Bipolaris Oryzae from Rice (Oryza sativa) by RAPD-PCR

Basavaraju Archana, Kukandhuru Ramachandra Kini, and Harishchandra Sripathy Prakash
Department of Studies in Biotechnology, Asian Seed Health Centre, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India
Abstract—Rice (Oryza sativa L.) is a major food for India as well as the world population. Brown spot caused by heterothallic ascomycete Cochliobolus miyabeanus (Anamorph: Bipolaris oryzae (Breda de Haan). Shoemaker is an important constraint to rice production in most rice growing environments. The pathogen also causes leaf spot diseases on several graminaceous hosts, but is considered host specific. Bipolaris oryzae is notorious for its diversity and genetic variability. Understanding the variability of the fungal population associated with brown spot could improve disease control strategies. This study was done to generate genomic finger prints using RAPD markers as well as to find out genetic diversity in B. oryzae isolates collected from 12 different geographical regions of India. A total of 12 isolates and 40 RAPD primers were used to generate genomic fingerprint profile for B. oryzae. Cluster analysis using UPGMA method revealed polymorphism ranging between 50 to 91.66%. The number of loci produced from each marker ranged from 2-10 with a total of 212 loci from all isolates. The molecular weight of scorable loci ranged from 150 to 2500 bp. The pattern of RAPD bands could not show direct correlation between polymorphism and climatic or geographical areas. 

Index Terms—bipolaris oryzae, rice brown spot, RAPD, genetic linkage, dendrogram, polymorphism

Cite: Basavaraju Archana, Kukandhuru Ramachandra Kini, and Harishchandra Sripathy Prakash, "Study of Genomic Fingerprints Profile of Indian Bipolaris Oryzae from Rice (Oryza sativa) by RAPD-PCR," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 1, pp. 83-92, January 2014.
Copyright © 2012-2015 International Journal of Life Sciences Biotechnology and Pharma Research, All Rights Reserved