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Day to Day Analysis of Amylase and Trehalase Activity in the Haemolymph of Silkworm Bombyx mori L. Infected with Fungal Pathogen Beauveria bassiana (BALS.) Vuill

K Rajitha and G Savithri
Department of Sericulture, S P Mahila Visvavidyalayam (Women's University), Tirupati -517502, Andhra Pradesh, India
Abstract—Increased trend of amylase activity was recorded in the haemolymph of healthy larvae up to the 5th day (0.475 mg/ml to 0.667 mg/ml) of the instar and then the enzyme activity was declined on the 6th day (0.626 mg/ml). In experimental larvae the enzyme activity was elevated up to 3rd day (0.462 mg/ml to 0.523 mg/ml) and then the activity of the enzyme was declined significantly (0.490 mg/ml to 0.467 mg/ml) during the rest of the instar. Whereas the activity of trehalase was increased in haemolymph of 5th instar silkworm from the 1st day to 6th day (1.49 mg/ml to 2.97 mg/ml). In comparison to control the trehalase activity shows a negligible increase up to 3rd day (1.5 mg/ml to 1.76 mg/ml) of the inoculated 5th instar larvae then the enzyme activity was declined significantly during the rest of the instar (1.64 mg/ml to 1.44 mg/ml). 

Index Terms—bombyx mori, beauveria bassiana, haemolymph, amylase and trehalase

Cite: K Rajitha and G Savithri, "Day to Day Analysis of Amylase and Trehalase Activity in the Haemolymph of Silkworm Bombyx mori L. Infected with Fungal Pathogen Beauveria bassiana (BALS.) Vuill.," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 1, pp. 225-230, January 2014.
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