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Highly Pathogenic Avian Influenza Virus H5N1 Neuraminidase Expressed in SF9 Insect Cells with a Baculovirus Free System

Majid Jamshidian Mojaver1,5, Mohammad Reza Bassami2,3,5, Hesam Dehghani1,4,5, and Mohammad Hasan Bozorgmehri Fard6
1. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
3. Institute of Poultry , Ferdowsi University of Mashhad, Mashhad, Iran
4. Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
5. Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
6. Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Iran
Abstract—This article reports heterologous expression in Sf9 insect cells of N1 neuraminidase derived from avian influenza virus A/chicken/Iran/53-3/2008(H5N1). A gene encoding the neuraminidase N1 was fused directly in-frame with the adipokinetic hormone (AKH) secretion signal and 6xHis- Tag coding sequence upstream of the cloning site for expressing fusion recombinant N1 protein with N-terminal tags in pIEx-3 vector. Recombinant N1 neuraminidase was expressed in Sf9 insect cells as a 80 kDa secreted protein. This insect-based Baculovirus-Free heterologous expression system provided functionally recombinant N1 neuraminidase that should be useful in anti-influenza drug screening, detection and diagnostic tests and also as a potential protein- based vaccine. 

Index Terms—highly pathogenic avian influenza, neuraminidase (NA), H5N1, heterologous expression, Sf9 insect cells, baculovirus-free

Cite: Majid Jamshidian Mojaver, Mohammad Reza Bassami, Hesam Dehghani, and Mohammad Hasan Bozorgmehri Fard, "Highly Pathogenic Avian Influenza Virus H5N1 Neuraminidase Expressed in SF9 Insect Cells with a Baculovirus Free System," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 2, pp. 51-56, April 2014.
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