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In Vitro Propagation of Acalypha Indica Linn.: A Medicinally Important Plant

Mousmi Saikia and Pratap J handique
Department of Biotechnology, Gauhati University, Guwahati-781014, Assam, India
Abstract—An in vitro regeneration protocol for Acalypha indica was developed using nodal explants on MS medium augmented with different concentrations and combinations of plant growth regulators for direct and indirect regeneration. The highest frequency (63.2%) of multiple shoot regeneration with maximum number of shoots (4.1shoots per explants) was noticed on ½ strength MS medium supplemented with BAP (1.0 mg/L) in combination with KIN (0.5 mg/L). Highest percentage of callus induction (81.5%) from nodal segments was observed on ½ strength MS medium supplemented with 2, 4-D (1.5 mg/L). The in vitro derived calli were sub-cultured for shoot regeneration. ½ strength MS medium fortified with BAP (1.0 mg/L) in combination with NAA (0.5 mg/L) showed the highest percentage (62.8%) shoot proliferation from the nodal segments derived calli. Elongated shoots were rooted best on ½ strength MS medium containing IBA (1.0 mg/L) producing maximum number of roots with 70% response. The plantlets were gradually acclimatized and successfully transferred to field condition with 100% survival rate within 6 weeks after rooting. The standardized protocol reported in this study may help in large scale propagation of this plant species which is currently exploited from the nature. 

Index Terms—acalypha indica, in vitro propagation, calli, nodal segment, regeneration

Cite: Mousmi Saikia and Pratap J handique, "In Vitro Propagation of Acalypha Indica Linn.: A Medicinally Important Plant," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 2, pp. 85-93, April 2014.
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