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Setaria Cervi Antigen Fraction for Serodiagnosis of Human Lymphatic Filariasis by Dot-ELISA

Priyanka Priyadarshi1, Deep C Kaushal2, Ashish Tandon1, Shakir Ali3, and Nuzhat A Kaushal1
1. Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow-226031, India
2. Amity University Uttar Pradesh, Lucknow Campus, Lucknow-226028, India
3. Department of Biochemistry, Jamia Hamdard, New Delhi-110062, India
Abstract—A partially purified antigen fraction of Setaria cervi was used in Dot-enzyme linked immunosorbent assay (Dot-ELISA) for detection of filaria-specific antibodies in Wuchereria bancrofti infected patient sera. The S. cervi adult antigen extract was fractionated by gel filtration on Sephacryl S-500 column and antigen fractions (obtained just after the void volume) showed high reactivity with polyclonal antibodies against S. cervi excretory-secretory antigen fraction-I (having potential for immunodiagnosis of human filariasis) as well as with filarial patient serum pools. The partially purified S. cervi antigen fraction (ScAS-1) was used in Dot-ELISA for detection of antibodies in filarial patient sera. The Dot-ELISA was optimized in terms of optimum antigen concentration, antibody dilutions and secondary antibody conjugate and 50 ng of antigen per dot, serum dilution of 1:250 and 1:1000 dilution of secondary antibody conjugate were found optimal for Dot-ELISA. The optimized Dot-ELISA was evaluated using 30 filarial patients sera, 20 sera with other parasitic infections and 20 normal healthy individuals. The partially purified S. cervi antigen fraction could detect anti-filarial antibodies in filarial patients sera by Dot-ELISA and thus can be used for serodiagnosis of human filariasis. 

Index Terms—filariasis, bovine filarial parasite, diagnosis, dot-ELISA, setaria cervi

Cite: Priyanka Priyadarshi, Deep C Kaushal, Ashish Tandon, Shakir Ali, and Nuzhat A Kaushal, "Setaria Cervi Antigen Fraction for Serodiagnosis of Human Lymphatic Filariasis by Dot-ELISA," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 2, pp. 108-115, April 2014.
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